RNA

Part:BBa_K206030:Design

Designed by: Amelia Hardjasa and Alex Ng   Group: iGEM09_British_Columbia   (2009-05-11)


Lock 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence provided in the supplementary table of Isaacs paper is lacking 5 bases present in the diagram that appear to contribute to secondary structure folding. These bases were included in the sequence, with one A->T change to avoid the inclusion of an EcoRI site.


Source

Taken from Isaacs et al., with modifications to remove an extra EcoRI site.

References